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Andrographis paniculata containing andrographolide measuring method which measure
[Differential] (1) The product leaf cross-section: the square or rectangular type epidermal cells, epidermal cells under the small upper and lower epidermis are round cells containing crystals, oblong or rod-shaped limestone body; and a gland scales, some visible non-glandular hairs. Palisade tissue is l ~ 2 rows of cells, throughout the top of the main vein; loosely arranged spongy tissue. Main vein vascular bundle outside the tough type, showing the groove-like, above the xylem also crystal cells.
Leaf surface view: upper and lower epidermis has increased crystal cell, containing a large spiral-shaped cystoliths, to a diameter of about 36μm, about to 180μm, the larger end of umbilical samples mark, ripple-like layer. Under stomatal clouds, straight shaft, subsidiary cells size disparity, there are infinitive. Adeno-scale spherical head flat, 4, 6 (8) cells, diameter to 40μm, handle very short. Non-glandular hairs 1 to 4 cells, about to 160μm, to base diameter of about 40μrn, horny surface texture.
(2) take Andrographis control medicine 0.5g, plus ethanol 30ml, ultrasonic treatment for 30 minutes, filtered, and the filtrate was concentrated to 5ml, as the reference drug solution. Then take dehydroandrographolide reference, andrographolide reference substance, add ethanol made from a mixed solution each containing lmg per lml, as the reference solution. Thin layer chromatography (Appendix VIB) test, to learn [determination] under the test solution, the reference solution and each 6μl said medicine solution 4μl, point in the same silica gel GF254 TLC plate with trichloro methane - ethyl acetate - methanol (4: 3: 0.4) as the agent, started out, drying, UV light (254nm), under review. Test products for chromatography, chromatography with the reference drug and the reference substance chromatography corresponding position, was the same color spots respectively; sprayed with a 2% ethanol solution of 3,5-dinitro-benzoic acid -2mol / L potassium hydroxide solution (1: 1) mixed solution (formulated before use), and immediately view in daylight. Test products for chromatography, chromatography with the reference drug and the reference substance chromatography corresponding position, respectively, was the same color spots.
[Check] Ye shall not be less than 30%.
[Extract] according to alcohol-soluble extract assay (Appendix ⅩA) was measured under the hot dip method, using ethanol as solvent, not less than 8.0%.
[Content determination of high-performance liquid chromatography (Appendix VID) was measured.
Chromatographic conditions and system suitability test octadecylsilane bonded silica as a filler; methanol-water (52:48) as the mobile phase; detection wavelength of andrographolide 225nm, dehydroandrographolide detection wavelength of 254nm. Number of theoretical plates and andrographolide dehydroandrographolide peak calculation should be not less than 2000.
Reference solution of reference taking andrographolide, dehydroandrographolide reference standard amount, accurately weighed, add methanol produced per lml each containing 0.1mg of mixed solution, that is, too.
Preparation of the test solution to take the product powder (sieve through IV) of about 0.5g, accurately weighed, set stoppered Erlenmeyer flask, precision by adding 40% methanol 25ml, said that given the weight, soak 1 hour, sonication ( power 250W, frequency 33kHz) 30 minutes, let cool, then weighed and the weight, with 40% methanol to complement the weight loss, shaking, filtration. Precise amount of filtrate 10ml, set a neutral alumina column (200 to 300 mesh, 5g, inner diameter of 1.5cm), the elution with methanol 15ml, collecting the eluent, set 50ml volumetric flask, add methanol to the mark, shake, that was.
Determination of Precision learn from each 5μl of the test solution the reference solution and into the liquid chromatograph to measure, that is, too.
This product calculated on dry goods, including the total amount of andrographolide (C20H30O5) and dehydroandrographolide (C20H28O4) not less than 0.80%.
[Cooked] to remove impurities, washed, cut and dry.
This product was irregular segments. Square cylindrical stems, nodes slightly swollen. Section is not flat, with white pith. Leaves more wrinkled or broken, with complete flattening was lanceolate or ovate-lanceolate, apex Zhejiang, base cuneate decurrent, entire or undulate; upper surface green, lower surface gray-green, smooth on both sides. Gas micro, taste very bitter.
Identification (except leaf cross-section) with herbs.
[Sexual flavor Meridian] and bitter cold. Heart, lung, colon, bladder.
Functions and Indications] detoxification, cooling, swelling. For colds, fever, sore throat, mouth sores, Dayton labor cough cough, diarrhea, dysentery, hot shower astringent pain, carbuncle swollen sore, Snake bites.
[Usage and dosage] 6 ~ 9g. Topical amount.
[Storage] home and dry place.